Engineer job offer “Methodological developments for high-throughput plant proteomics”

• Location: Plateforme d’Analyse Protéomique de Paris Sud-Ouest (PAPPSO), Gif-sur-Yvette (France)
• Date: 12 months contract from february 2023
• Gross salary: 2100-2500 € per month depending on the experience

Key words: plant proteomics, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), methodological development, data independent analysis, microflow

Context:

PAPPSO (www.pappso.inrae.fr) is a technological facility that aims to provide research teams with high skills and new-advanced equipments in the field of proteomics. For the last ten years, PAPPSO has been receiving more and more frequent requests for the analysis of large cohorts of plant samples (> 100 samples). These requests are all related to research projects in plant breeding or agro-ecology, where methodological approaches necessitating large experimental designs are increasingly turning to the exploitation of omics data collected downstream of the genome. To adress this evolution, PAPPSO needs to develop a new type of analysis in the field of high-throughput plant proteomics.

Some changes since the last announced version 0.4.57 :

• Huge performance enhancement of our TimsTOF raw data reader
• MassChroQ is now shipped in the Windows package
• Better integration of R and MCQR

Huge performance enhancement of our TimsTOF raw data reader

The TimsTOF raw data reader inside i2MassChroQ is now fully paralelized and uses an agressive cache system. On the same computer, previous version did the conversion of one Hela sample (120 minutes) in 1h10 and the current version now does it in 21 minutes using 4 threads. The same sample converted by ProteoWizard using 4 threads last 35 minutes. Identification results given by i2MassChroQ are better than same samples converted by ProteoWizard.

PROTÉOME VERT Days on June 9 and 10, 2022

IDEEV, Université Paris-Saclay, 12 route 128, 91190 Gif-sur-Yvette Rachel Carson room

The Data Management Plan of the facility is now available on Opidor !

PAPPSO’s Data Management Plan.

The PAPPSO users’ scientific council will take place on May 16 and will be open to all, users of the facility or interested by its activities. This half-day of information and presentations will be available in person at IDEEV (12 route 128, 91190 Gif-sur-Yvette, Rosalind Franklin room on the first floor) or by video via the following link (https://orsay.bbb.cnrs.fr/b/lan-l7m-axe-ais)